[Objective] This study aimed to investigate the sporulation conditions of Tri- choderma reesei by solid fermentation. [Method] With sporulation yield as the response value, single-factor test, Plackett-Burmam design, steepest ascent test, BoxBehnken design and response surface analysis were employed to optimize the con- ditions for sporulation of Trichoderma reesei by solid fermentation. [Result] Based on single-factor test, the most appropriate carbon source for Trichoderma reesei was straw stalk powder and wheat bran with the ratio of 3：2 and optimal amount of 15 g/L; the most appropriate inorganic nitrogen was （NH4）2O4 with the optimal amount of 3 g/L. According to Plackett-Burmam design, moisture content, initial pH and incubation temperature were identified as significant factors affecting the sporulation yield of Trichoderma reeseL The maximum sporulation yield area was approached by steepest ascent test. Based on Box-Behnken design and response surface analysis, the optimal fermentation conditions for the maximum sporulation yield were determined as： straw stalk powder of 6 g/L, wheat bran of 9 g/L, （NH4）2SO4 of 3 g/L, moisture content of 65%, incubation temperature of 29 ℃, fermentation period of 72 h and initial pH of 5.5, under these conditions, the sporulation yield reached 2×10^10 spores/g, which was improved by 1.4 times compared with that before optimization. [Conclusion] In this study, the conditions for sporulation of Trichoderma reesei by solid fermentation were optimized with low-cost straw stalk powder and wheat bran as carbon sources, which was conducive to reducing the production cost of Trichoderma reesei and increasing the sporulation yield, showing certain social and economic significance.
