[Objective] This study aimed to investigate the effects of different pretreat- ments on enzymatic saccharification of Miscanthus sinensis and improve reducing sugar yield in the enzymolysis process. [Method] M. sinensis was pretreated with 60Co y-ray irradiation and alkaline hydrogen peroxide, to analyze their effects on re- ducing sugar yield of enzymatic hydrolysis. [Result] After pretreatment with 400 kGy 60Co y-ray irradiation, reducing sugar yield in the enzymolysis process of M sinensis was 76.24 mg/g; after synergic pretreatment with 400 kGy 60Co y-ray irradiation and alkaline hydrogen peroxide, reducing sugar yield in the enzymolysis process of M. sinensis was 505.08 mg/g, which was improved by 5.6 times compared to that in pretreatment with 400 kGy 60Co y-ray irradiation. Based on process optimization, the optimal hydrolysis conditions were obtained： pretreatment temperature 30 ℃, NaOH concentration 1.2%, hydrogen peroxide concentration 2%, pretreatment time 6 h. [Conclusion] Synergic pretreatment with 60Co y-ray irradiation and alkaline hydrogen peroxide could significantly improve reducing sugar yield in the enzymolysis process of M. sinensis, which provided a new theoretical basis for preparing fuel ethanol with M. sinensis.
