[Objective] The aim was to study rooting environment of strawberry ＂Bechi- ka＂ seedlings, and the somaclone domesticated transplanting was carded on. [Method] With the stem tip of stolon as material, the influence factom of rooting and the effects of different transplanting substrata on survival rate was investigated. [Result] The results showed that： 1/2MS was the best concentration for rooting and the rooting rate could reach 100%. IBA was better than IAA for rooting on growth regu- lator levdls, and it had the best effect on rooting with 0.3 mg/L, which produced the thickest and most-fibrous roots. Activated carbon had positive influences on the growth of roots, and the suitable concentration of activated carbon was 0.5 mg/l_ for rooting. The study found that survival rate of tissue culture seedlings was obviously higher in the combination of perlite and vermiculite than that in the combination of humus and sand, and the survival rate could reach 98.1% （perlite：vermiculite=l：l）. Seedlings in various transplanting substrata could normally bloom, fruit and form stolons. [Conclusion] The study provided technical support for achieving large-scale production of virus-free strawberry and quickly cultivating good seedlings.
