Objective To understand three big and familiar interference things are concrete interference circumstances inbiochemistry examine and it's the dest eliminate way.Methods Three specimens of the serious hemolytic and the serious jaundice and the serious milk blood are different.diluted by physiology brine ( 0.9%NaCL ) and distilled water ( DH2O ) Every kind of items is examined under establishing the blank of sample and reagent , and analysis comparison result.To put a normal specimen into adopt artificial hemolytic.To determine potassium ( K ) and hemoglobin ( Hg ) in the serum of different hemolytic degree , and them analysis results. Results On OLYMPUS AU-400, when TG>37.04mmol/L and CHO>25.59mmol/L, T-BIL>398.7~tmol/L and D- BIL>60.81μmol/L and Hg>51.2g/L, the results can not be detected.Most items have comparison relation between It' s 1 : 3 to 1 :8.But TBA Lpa is 1 : 4 to l : 6 and Ca P is l : 1.5 to l : 2.5.Determining results have not comparison relation when the dilute times are larger than 1 : l0 and smaller than 1 : 3.Both results have not significant difference within having comparison relation after the specimen is diluted by 0.9%NaCl and distilled water ( P>0.05 ).But Lpa has significant difference( P<0.05 ).On BeckmCX3o The results an of Na and Cl have significant difference after the .specimen is diluted by 0.9% NaCl and distilled water ( P<0.05 ) .When the serious jaundice and the serious milk blood is diluted by distilled water, results of K Na CI CO2 BUN have not significant difference ( P>0.05 ) .So is Na Cl CO2 BUN Ca Cre for the serious hemolytic specimen .But Ca Cre and K have significant difference ( P<0.05 ) .The relativity of Hg and K are good in serum after a normal specimen is artificial hemolytic (r=-0.9999) .Comparing with determining results and formula calculating results of K have not significant difference ( P>0.05 ) .Conclusion Three kinds of familiar interference substances havs interference for biochemistry examining.Different instrument and reagent and methods cause different
