盆底功能障碍性疾病是我国女性常见疾病,其发病率随年龄逐年上升,由于盆底肌松弛等原因,常导致女性出现盆腔脏器脱垂、排尿排便功能障碍、性功能障碍等一系列问题,严重影响妇女的生活质量。低频电刺激作为一种非侵入性、安全可靠的治疗方法,在治疗女性盆底功能障碍性疾病中起到重要作用,其治疗效果显著,本文将有关低频电刺激在女性盆底功能障碍性疾病中的应用予以综述。Pelvic floor dysfunction disease is a common disease among women in China, and its incidence rate increases with age. Due to pelvic floor muscle relaxation and other reasons, it often leads to a series of problems such as pelvic organ prolapse, urinary and defecation dysfunction, sexual dysfunction, etc., which seriously affects women’s quality of life. As a non-invasive, safe and reliable treatment method, low-frequency electrical stimulation plays an important role in the treatment of female pelvic floor dysfunction diseases, and its therapeutic effect is remarkable, this paper reviews the application of low-frequency electrical stimulation in female pelvic floor dysfunction diseases.
目的探讨线粒体动力相关蛋白1(dynamic-related protein 1,DRP1)在肝癌细胞糖代谢重编程中的作用。方法(1)siRNA下调肝癌细胞SNU-739中DRP1表达后,检测对肝癌细胞葡萄糖摄取与乳酸产生的影响,以明确DRP1对肝癌细胞糖酵解的调控作用。(2)siRNA下调肝癌细胞SNU-739中DRP1表达后,检测对肝癌细胞氧耗速率与ATP产生的影响,以明确DRP1对肝癌细胞氧化磷酸化的调控作用。(3)siRNA下调肝癌细胞SNU-739中DRP1表达后,利用质谱检测对糖酵解与线粒体三羧酸循环代谢产物的影响。结果(1)下调DRP1可显著抑制肝癌SNU-739细胞的葡萄糖摄取(siCtrl:si-DRP1#1:si-DRP1#2=1.000±0.069:0.417±0.032:0.400±0.040;F=141.400,P<0.001)与乳酸产生(siCtrl:si-DRP1#1:si-DRP1#2=1.000±0.050:0.327±0.040:0.310±0.036;F=256.700,P<0.001)。(2)下调DRP1可激活肝癌SNU-739细胞的氧耗速率(siCtrl:si-DRP1#1:si-DRP1#2=1.000±0.069:1.623±0.081:1.591±0.046;F=81.720,P<0.001)与ATP产生(siCtrl:si-DRP1#1:si-DRP1#2=1.000±0.062:1.813±0.093:1.850±0.070;F=119.200,P<0.001)。(3)下调DRP1后,肝癌SNU-739细胞中糖酵解中间产物葡萄糖、丙酮酸与乳酸的水平均显著降低(均P<0.001),而三羧酸循环关键产物柠檬酸、延胡索酸与苹果酸的水平均显著升高(均P<0.001)。结论线粒体动力相关蛋白DRP1通过激活糖酵解并抑制氧化磷酸化而促进肝癌细胞的糖代谢重编程。
