Objective To observe the expression of HSP70 mRNA in the rat induced by glutamine.Methods ①Twenty four healthy Sprague-Dawley rats were randomly divided into four groups（n=6 in each group）: groupⅠ received saline i.v.;group Ⅱ received glutamine（Gln） 0.15 g·kg-1 i.v.;group Ш received Gln 0.45 g·kg-1 i.v.;group Ⅳ received Gln 0.75 g·kg-1 i.v..All rats were killed after 4 h administration of Gln.HSP70 mRNA from heart、liver、lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.② Thirty healthy Sprague-Dawley rats were randomly divided into five groups（n=6 in each group）.They were killed at 1 h（group 1 h）、6 h（group 6 h）、12 h（group 12 h）、24 h（group 24 h） and 48 h（group 48 h） after administration of Gln(0.75 g·kg-1).HSP70 mRNA from heart、liver、lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.Results ① The expression of HSP70 mRNA in group Ⅱ、Ш and Ⅳ were higher than groupⅠ（P<0.05）,and the expression in groupⅡ、Ш and Ⅳ increased gradually.②The expression of HSP70 mRNA in group 1 h、 6 h and 12 h was higher than that in 24 h and 48h（P<0.05）.Conclusion Glutamine induces the expression of HSP70 mRNA in rats,and it occurs with the manner of dose and time dependent.
