Subcellular location is one of the key biological characteristics of proteins. Position-specific profiles （PSP） have been introduced as important characteristics of proteins in this article. In this study, to obtain position-specific profiles, the Position Specific lterative-Basic Local Alignment Search Tool （PSI-BLAST） has been used to search for protein sequences in a database. Position-specific scoring matrices are extracted from the profiles as one class of characteristics. Four-part amino acid compositions and lst-7th order dipeptide compositions have also been calculated as the other two classes of characteristics. Therefore, twelve characteristic vectors are extracted from each of the protein sequences. Next, the characteristic vectors are weighed by a simple weighing function and inputted into a BP neural network predictor named PSP-Weighted Neural Network （PSP-WNN）. The Levenberg-Marquardt algorithm is employed to adjust the weight matrices and thresholds during the network training instead of the error back propagation algorithm. With a jackknife test on the RH2427 dataset, PSP-WNN has achieved a higher overall prediction accuracy of 88.4% rather than the prediction results by the general BP neural network, Markov model, and fuzzy k-nearest neighbors algorithm on this dataset. In addition, the prediction performance of PSP-WNN has been evaluated with a five-fold cross validation test on the PK7579 dataset and the prediction results have been consistently better than those of the previous method on the basis of several support vector machines, using compositions of both amino acids and amino acid pairs. These results indicate that PSP-WNN is a powerful tool for subcellular localization prediction. At the end of the article, influences on prediction accuracy using different weighting proportions among three characteristic vector categories have been discussed. An appropriate proportion is considered by increasing the prediction accuracy.
项目式学习是一种积极的教学方式,具有强调能力培养、激发学习兴趣、提高官兵参与度等优点。基于我军军事职业教育的本质内涵,本文按照构建军事职业教育岗位能力素质模型、新型资源体系、在线学习项目三个步骤,以《“悦”读一本书》为例探索实践了军事职业教育项目式学习,以期为军事职业教育事业建设发展提供理论与实践参考。Project-based learning is an active teaching method, which has the advantages of emphasizing ability cultivation, stimulating learning interest and enhancing officers’ participation. Based on the essential connotation of military continuing education, this paper explores and practices project-based learning in accordance with the three steps of constructing military continuing education post ability quality model, new resource system and online learning project, taking “‘Happy’ reading a Book” as an example. This paper will provide theoretical and practical reference for the construction and development of military continuing education.
