目的:通过菟丝子含药血清对肿瘤坏死因子-α(TNF-α)诱导人体外培养蜕膜细胞凋亡的影响,以期从蜕膜细胞凋亡角度探讨菟丝子可能的安胎机制。方法:体外培养人蜕膜细胞,通过TNF-α诱导蜕膜细胞凋亡,菟丝子含药血清干预,MTT比色实验分析TNF-α及菟丝子含药血清作用后人蜕膜细胞活力,并用流式细胞术分析菟丝子含药血清对人蜕膜细胞凋亡的影响。结果:TNF-α作用后的人蜕膜细胞活性均低于空白对照组,随着TNF-α质量分数的增加,活性越来越小;10,50μg/L的TNF-α作用后,与空白对照组对比,差别有统计学意义(P<0.05)。200 m L/L菟丝子含药血清干预后的人蜕膜细胞活性高于TNF-α凋亡模型组,差别有统计学意义(P<0.05)。流式细胞术示菟丝子含药血清作用后的人蜕膜细胞凋亡率低于凋亡模型组,差别有统计学意义(P<0.05)。结论:TNF-α能降低体外培养人蜕膜细胞活力,诱导细胞凋亡;菟丝子含药血清对TNF-α诱导培养的人蜕膜细胞凋亡有一定抑制作用。
目的:本研究旨在利用知识图谱可视化技术,深入分析寿胎丸研究的现状、热点和成果,为未来相关领域的研究提供重要参考。方法:在中国知网中以关键词“寿胎丸”为检索主题,搜索时间范围为2004年1月1日至2023年12月31日,筛选相关文献,使用CiteSpace 6.3.R1测量学分析文献,对相关知识图谱进行绘制和分析。结果:检索到596篇文章,其中以雷磊为发文量最多的作者,湖南中医药大学为发文量最多的机构;寿胎丸相关研究的高频关键词有:寿胎丸,先兆流产,孕激素,胎动不安等;重点关键词聚类有21类。结论:寿胎丸的研究热点在实验研究和临床应用上,联合应用治疗是今后研究趋势。Objective: This study aims to study is to deeply analyze the current status, hotspots and achievements of Shoutai Wan research by using knowledge graph visualization technology, which will provide an important reference for future research in related fields. Methods: In CNKI, the keyword “Shoutai Wan” was used as the search topic, and the search time was from January 1, 2004 to December 31, 2023. Relevant literature was screened, and the literature was analyzed by using CiteSpace 6.3.R1 measurements, and the relevant knowledge maps were drawn and analyzed. Results: 596 articles were retrieved, in which Lei Lei was the author with the largest number of articles, and Hunan University of Traditional Chinese Medicine was the institution with the largest number of articles;high-frequency keywords for Shoutai Wan-related research included Shoutai Wan, preeclampsia, progesterone, and fetal restlessness, etc.;the keyword focus clustered in 21 categories. Conclusion: The research hotspot of Shoutai Wan is in experimental research and clinical application, and the combined application of treatment is the future research trend.
