This study aimed to explore artificial propagation techniques for Glyptosternum maculatum that is endemic in the Yarlung Zangbo River. Total 86 female fish in weight of 0.065-0.250 kg were selected, and the average weight of every 15 fish was 0.30 kg. Oxytocic drugs were injected into the base of pectoral fins of the female fish. The results showed that the total amount of female fish with artificial insemination was 59. The gloss weight of the fertilized eggs was 897.5 g with total amount of 57 440, thus the average fecundity was 1 194 eggs/fish. The fecundity showed a positive correlation with body weight （P〈0.01）. The average induction ratio and fertilization rate were 69% and 73.5%, respectively. The optimum water temperature for hatching of the fertilized eggs was 13-14 ℃ with dissolved oxygen of 6.0- 7.2 mg/L. The accumulated temperature for embryonic development of G. maculatum ranged from 2 592 to 2 916℃·h. This set of completely artificial propagation techniques has a very important significance for the artificial breeding of G. maculatum.
