研究覆盆子多酚的大孔树脂富集工艺,并评价覆盆子多酚的生物活性。通过静态吸附–解吸试验选择适合富集覆盆子多酚的大孔树脂,拟合吸附方程判断大孔树脂的吸附动力学模型,以大孔树脂对覆盆子多酚粗提物中多酚的吸附量和解吸率为指标,对大孔树脂的上样液pH、多酚上样量、解吸液乙醇体积分数进行单因素试验,并通过正交试验确定大孔树脂富集覆盆子多酚的最优条件组合;评价富集后覆盆子多酚的纯度、对1,1-二苯-2-苦肼基(DPPH·)清除率以及对黄嘌呤氧化酶和α-葡萄糖苷酶的抑制活性,通过分子对接评价覆盆子主成分鞣花酸与黄嘌呤氧化酶、α-葡萄糖苷酶的结合能力。结果显示,AB-8大孔树脂最适合富集覆盆子多酚且其吸附覆盆子多酚的过程符合准二阶动力学模型,AB-8大孔树脂在上样液pH为3、多酚上样量为30 mg/g树脂、解吸液的乙醇体积分数为90%时,制备的覆盆子多酚的得率最高,达到85.36 ± 0.78%,富集后覆盆子多酚纯度从23.81 ± 1.75%上升到68.58 ± 0.60%,对DPPH·的清除率以及对黄嘌呤氧化酶和α-葡萄糖苷酶的抑制率的IC50分别为59.09 ± 0.42 μg/mL、97.71 ± 2.75 μg/mL、3.98 ± 0.43 μg/mL,分子对接结果也显示鞣花酸化合物与黄嘌呤氧化酶和α-葡萄糖苷酶有较好的结合能力。本研究为覆盆子多酚的工业化生产和开发利用提供理论参考。The study was conducted to optimize the adsorption process of polyphenols from raspberry (Rubus chingii Hu) on macroporous resin and evaluate the biological activity of polyphenols from raspberry . The macroporous resin suitable for adsorbing raspberry polyphenols was selected through static adsorption-desorption experiments, and the adsorption kinetic model was determined by fitting the adsorption equation. The adsorption capacity and desorption rate of polyphenols in crude extract of raspberry polyphenols by macroporous resin were used as indicators, and
