The electrochemical behavior of catechol(QH2) was studied on glassy carbon electrode activated by cyclic voltammetry.In KH2PO4-Na2HPO4 buffer solution of pH=6.0,a couple of sensitive redox peaks were recorded about 0.18V in the cyclic voltammograms.The resulting electrode showed excellent electrocatalytic activity for the oxidation of catechol,making oxidation peak potential shife more negatively.The catalytic oxidation current of catechol was linear with its concentration in the range of 5.0×10-6～1.0×10-5mol·L-1.The correlative coefficient was-0.99567.The detection limit was 1.2×10-6mol·L-1.Moreover,the activated glassy carbon electrode exhibited high sensitivity and good reproducibility.
A novel fluorimetric method is developed for the rapid determination of ribonucleic acid(yRNA)with salicylic acid(SA)as a fluorescence probe.It is based on the fluorescence enhancing of SA in the presence of yRNA with maximum excitation and emission wavelength at 299nm and 413nm respectively.Variables influencing fluorimetric response of the interaction between SA and yRNA,such as pH value and reaction time,are explored and optimized.Under optimal conditions,the calibration graph for yRNA is linear over the range of 1.0×10-6mol/L～5.0×10-5mol/L.The disturbance experiment is also studied.The corresponding detection limit is 1.0×10-7mol/L.The method is more sensitive and selective than currently applied techniques.
