本文针对传统生物化学教学模式中存在的问题,探讨了“基因工程制备乙肝疫苗虚拟仿真实验”课程的构建与实践。该课程以基因工程制备乙肝疫苗为核心,将现代生物技术与三维信息技术深度融合,构建了高度仿真的虚拟实验环境。文章详细阐述了该课程的教学理念、目标、内容、实施方式和考核标准。通过以学生为中心的教学设计,创新实验方案、教学方法和评价体系,旨在培养学生分析和解决复杂生物医学工程问题的能力,同时拓宽学生的思路和视野。This paper addresses the issues in traditional biochemistry teaching models and explores the construction and practice of the “Virtual Simulation Experiment on the Preparation of Hepatitis B Vaccine by Genetic Engineering” course. Centered on the preparation of hepatitis B vaccine through genetic engineering, the course integrates modern biotechnology with three-dimensional information technology to create a highly realistic virtual experimental environment. The paper elaborates on the teaching philosophy, objectives, content, implementation methods, and assessment criteria of the course. By adopting a student-centered teaching design, the course innovates experimental schemes, teaching methods, and evaluation systems, aiming to cultivate students’ abilities to analyze and solve complex biomedical engineering problems while broadening their perspectives.