An Buthus martensii Karsch Insect Toxin (BmK IT ) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1×107 PIBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8h) during a 192h in-fection.
The scorpion venoms are a rich source of toxic peptides.BmK IT is an excitatory scorpionβ-toxin in Buthus mart...
Renjia Yang Jun Du Yuejun Fu Aihua Liang (Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Biotechnology,Shanxi University,Taiyuan,030006,PR China)
Gene therapy is proposed as a novel therapeutic strategy for glioma.Adenovirus-mediated delivery of the condit...
Jun Du~a,Yuejun Fu~a,Jianing Wang~b,Renjia Yang~a and Aihua Liang~a (a Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Biotechnology,Shanxi University,Taiyuan 030006,PR China b Institute of Clinical Medicine,People's Hospital,Shiyan 442000,PR China)
