With the rapid development of economy and increase of population in the drainage areas, the nutrient loading has increased dramatically in the Changjiang estuary and adjacent coastal waters. To properly assess the impact of nutrient enrichment on phytoplankton community, seasonal microcosm experiments were conducted during August 2010-July 2011 in the coastal waters of Zhejiang Province. The results of the present study indicated that the chl a concentration, cell abundance, diversity indices, species composition and community succession of the phytoplankton varied significantly with different N/P ratios and seasons. Higher growth was observed in the 64：1 （spring）, 32：1 （summer）, 16：1 （autumn） and 128：1,256：1 （winter） treatments, respectively. The values of Shannon-Wiener index （H） and Pielou evenness index （J） were lower in the 8：1 and 16：1 treatments in autumn test, while H value was higher in the 128：1 and 8：1 treatments in winter test. A definite community succession order from diatoms to dinoflagel lares was observed in the autumn and winter tests, while the diatoms dominated the community throughout the culture in the spring and summer tests.
To explore the spatial-temporal distribution of the phytoplankton community and evaluate the combined effects of marine resource exploitation, net-collected phytoplankton and physical-chemical parameters were investigated in the Xiangshan Bay during the four seasons of 2010. A total of eight phyla, 97 genera, and 310 species were found, including 232 diatom species, 45 dinoflageUate species and 33 other taxa. The phytoplankton abundances presented a significant （P〈0.001） seasonal difference with the average of 60.66x104 cells/m3. Diatoms （mainly consisting of Coscinodiscus jonesianus, Cerataulina pelagica, Skeletonema costatum, and genus Chaetoceros） dominated the phytoplankton assemblage in all seasons. We found great spatio-temporal variation in community composition based on the multidimensional scaling and similarity analysis. Canonical correspondence analysis show that temperature, nutrition, illumination, and salinity were the main variables associated with microalgal assemblage. Compared with the previous studies, an increase in phytoplankton abundance and change in the dominant species coincided with increased exploitation activities in this bay （e.g. operation of coastal power plants, intensive mariculture, tidal fiat reclamation, and industrial and agricultural development）. The present findings suggest that the government should exercise caution when deciding upon developmental patterns in the sea-related economy.
