The full-length cDNA of LeSGR1 was cloned from tomato by RT-PCR and RACE.The cDNA encoded a protein of 272 ami...
Zongli Hu 1,Lei Deng 1,Bo Yan 1,Yu Pan 1,Min Luo 1,Xuqing Chen 2,Tingzhang Hu 1 and Guoping Chen 1 (1 Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering College, Chongqing University,Chongqing 400044
The full-length cDNA of LeMBF1 was cloned from tomato by RT-PCR.The cDNA encoding an open reading frame of 139...
Zongli Hu 1,Lei Deng 1,Dafei Wang 1,Yu Pan 1,Yang Liu 1,Xuqing.Chen 2,Tingzhang Hu 1 and Guoping Chen 1 (1 Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering college, Chongqing University,Chongqing 400044
目的确定胚胎发育晚期丰富蛋白(LEA蛋白)在体外不同压力胁迫下对酶活性的保护作用,并对其可能机制进行探讨。方法评估Os LEA5蛋白对于防止乳酸脱氢酶(LDH)免受高温(43℃)、反复冻融和干燥脱水伤害的保护能力。磷酸盐缓冲液、牛血清蛋白(BSA)和纯水分别用于阳性和阴性对照。结果 Os LEA5蛋白组相对活性百分比在热处理10、20、30 min后分别是纯水对照组的2.2、3.7、5.4倍。在5个反复冻融循环后,纯水对照组的LDH活性降至7%甚至更低,而质量比2∶1和5∶1的Os LEA5蛋白组分别为28%和69%,质量比为10∶1组其活性几乎未受到影响。经过干燥处理后,不同质量比组LDH活性均有明显降低,其中纯水对照组和磷酸盐缓冲液组的LDH接近完全失活。质量比为5∶1和10∶1的Os LEA5蛋白组相对活性分别达24%和36%。结论 Os LEA5蛋白能够在各种环境胁迫下有效保护底物酶的体外活性或防止失活。
